代表取締役　王勇 (Wang, Yong)　理学博士、農学博士

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　​私は中国に生まれ、中国科学院海洋研究所で理学博士学位を取得しました。2000年、日本学術振興会の博士研究員として来日し、東京都立大学で海洋生物の胚発生に関与する遺伝子について研究を行いました。その後、経済産業省の産業技術総合研究所（AIST）と理化学研究所の筑波研究所でヒットやマウスのがん関連遺伝子についての研究を行いました。

　そこで私は自らの知識と技術を社会に還元すべく、農林水産省管轄下の農業環境技術研究所（現：農研機構・農業環境変動研究センター）に入り、土壌診断のために、土壌からRNAを抽出する技術を開発しました。さらに、土壌から抽出したDNAやRNAを用いて、定量PCR、マイクロアレイ及び次世代シークエンシング技術で土壌中における微生物の存在、種類、分布、環境因子の影響などを調べました。

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​​略歴：

中国　山東師範大学　生物学部　卒業

中国　山東師範大学大学院　動物学専攻　理学修士学位取得

中国科学院海洋研究所　海洋生物学専攻　理学博士学位取得

東京大学大学院　農学生命科学研究科　応用生命化学専攻　農学博士学位取得（論文博士）

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​発表した著作物（一部）：

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土壌微生物学分野

 

Yang W, Wang Y, Tago K, Tokuda S, Hayatsu M. (2017) Comparison of the effects of phenylhydrazine hydrochloride and dicyandiamide on ammonia-oxidizing bacteria and archaea in Andosols. Front. Microbiol. 8, 2226. doi: 10.3389/fmicb.2017.02226.

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Wang Y, Uchida Y, Shimomura Y, Akiyama H, Hayatsu M. (2017) Responses of denitrifying bacterial communities to short-term waterlogging of soils. Sci. Rep. 7, 803. doi: 10.1038/s41598-017-00953-8.

 

Hayatsu M, Tago K, Uchiyama I, Toyoda A, Wang Y, Shimomura Y, Okubo T, Kurisu F, Hirono Y, Nonaka K, Akiyama H, Itoh T, Takami H. (2017) An acid-tolerant ammonia-oxidizing γ-proteobacterium from soil. ISME J.11, 1130-1141.

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Akiyama H, Hoshino YT, Itakura M, Shimomura Y, Wang Y, Yamamoto A, Tago K, Nakajima Y, Minamisawa K, Hayatsu M. (2016) Mitigation of soil N2O emission by inoculation with a mixed culture of indigenous Bradyrhizobium diazoefficiens. Sci. Rep. 6, 32869. doi: 10.1038/srep32869.

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Uchida Y, Wang Y, Akiyama H, Nakajima Y, Hayatsu M. (2014) Expression of denitrification genes in response to a waterlogging event in a Fluvisol and its relationship with large nitrous oxide pulses. FEMS Microbiol. Ecol. 88, 407-423.

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Itakura M, Uchida Y, Akiyama H, Hoshino YT, Shimomura Y, Morimoto S, Tago K, Wang Y, Hayakawa C, Uetake Y, Sanchez C, Eda S, Hayatsu M, Minamisawa K (2013) Mitigation of nitrous oxide emissions from soils by Bradyrhizobium japonicum inoculation. Nature Clim. Change 3, 208–212.

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Wang Y, Nagaoka K, Hayatsu M, Sakai Y, Tago K, Asakawa S, and Fujii T. (2012) A novel method for RNA extraction from Andosols using casein and its application to amoA gene expression study in soil. Appl. Microbiol. Biotechnol. 96, 793-802.

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Iino T, Wang Y, Miyauchi K, Kasai D, Masai E, Fujii T, Ogawa N, Fukuda M. (2012) Specific gene responses of a gram-positive polychlorinated-biphenyl degrader, Rhodococcus jostii RHA1 during growth in soil. Appl. Environ. Microbiol. 78, 6954-6962.

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Okubo T, Tsukui T, Maita H, Okamoto S, Oshima K, Fujisawa T, Saito A, Futamata H, Hattori R, Shimomura Y, Haruta S, Morimoto S, Wang Y, Sakai Y, Hattori M, Aizawa S, Nagashima KV, Masuda S, Hattori T, Yamashita A, Bao Z, Hayatsu M, Kajiya-Kanegae H, Yoshinaga I, Sakamoto K, Toyota K, Nakao M, Kohara M, Anda M, Niwa R, Jung-Hwan P, Sameshima-Saito R, Tokuda S, Yamamoto S, Yamamoto S, Yokoyama T, Akutsu T, Nakamura Y, Nakahira-Yanaka Y, Takada Hoshino Y, Hirakawa H, Mitsui H, Terasawa K, Itakura M, Sato S, Ikeda-Ohtsubo W, Sakakura N, Kaminuma E, Minamisawa K. (2012) Complete genomic structure of Bradyrhizobium sp. S23321: insights into symbiosis evolution in soil oligotrophs. Microbes Environ. 27, 306-315.

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Wang Y, Morimoto S, Ogawa N, Fujii T. (2011) A survey of the cellular responses in Pseudomonas putida KT2440 growing in sterilized soil by microarray analysis. FEMS Microbiol. Ecol. 78, 220-232.

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Nazari B, Saito A, Kobayashi M, Miyashita K, Wang Y, Fujii T. (2011) High expression levels of chitinase genes in Streptomyces coelicolor A3(2) grown in soil. FEMS Microbiol. Ecol. 77, 623-635.

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Wang Y and Fujii T. (2011) Evaluation of methods of determining humic acids in nucleic acid samples for molecular biological analysis. Biosci. Biotechnol. Biochem. 75, 355-357.

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Wang Y, Morimoto S, Ogawa N, Oomori T, Fujii T. (2009) An improved method to extract RNA from soil with efficient removal of humic acids. J. Appl. Microbiol. 107, 1168-1177.

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Wang Y, Shimodaira J, Miyasaka T, Morimoto S, Oomori T, Ogawa N, Fukuda M, Fujii T. (2008). Detection of bphAa gene expression of Rhodococcus sp. strain RHA1 in soil using a new method of RNA preparation from soil. Biosci. Biotechnol. Biochem. 72, 694-701.

 

分子生物学分野

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Kakinuma  N, Roy BC, Zhu Y, Wang Y, Kiyama R. (2008) Kank regulates RhoA-dependent formation of actin stress fibers and cell migration via 14-3-3 in PI3K-Akt signaling. J. Cell Biol. 181, 537-549.

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Zhu Y, Kakinuma N, Wang Y, Kiyama R. (2008). Kank proteins: A new family of ankyrin-repeat domain-containing proteins. Biochim. Biophys. Acta 1780, 128-133.

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Wang Y, Kakinuma N, Zhu Y, Kiyama R (2006). Nucleo-cytoplasmic shuttling of human Kank protein accompanies intracellular translocation of β-catenin. J. Cell Sci. 119, 4002-4010.

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Wang Y, Onishi Y, Kakinuma N, Roy BC, Aoyagi T, Kiyama R (2005). Alternative splicing of the human Kank gene produces two types of Kank protein. Biochem. Biophys. Res. Commun. 330, 1247–1253.

 

海洋生物学分野

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Lang GH, Wang Y, Nomura N, Matsumura M (2004). Detection of telomerase activity in tissues and primary cultured lymphoid cells of Penaeus japonicus. Mar. Biotechnol. (NY). 6, 347-354. 

 

Wang Y, Zhang PJ, Yasui K, Saiga H (2002). Expression of Bblhx3, a LIM-homeobox gene, in the development of amphioxus Branchiostoma belcheri tsingtauense. Mech. Dev. 117, 315-319.

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Yasui K, Li G, Wang Y, Saiga H, Zhang P, Aizawa S (2002). β-catenin in early development of the lancelet embryo implicating a specific determination of the embryonic polarity. Dev. Growth Differ. 44, 467-475.

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Satoh G, Wang Y, Zhang P, Satoh N (2001). Early development of amphioxus nervous system with special reference to segmental cell organization and putative sensory cell precursors: a study based on the expression of pan-neuronal marker gene Hu/elav. J. Exp. Zool. 291, 354-364. 

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Yasui K, Saiga H, Wang Y, Zhang PJ and Semba I (2001). Early expressed genes showing a dichotomous developing domains in the lancelet embryo. Dev. Growth Differ. 43, 185-194.

 

総説

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Wang Y, Hayatsu M, Fujii T. (2012) Extraction of bacterial RNA from soil: challenges and solutions. Microbes Environ. 27, 111-121.

 

Kakinuma K, Zhu Y, Wang Y, Roy BC, Kiyama R. (2009) Kank proteins: structure, functions and diseases. Cell. Mol. Life Sci. 66, 2651-2659.

 

著書

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Wang, Y, Hayatsu, M., and Fujii, T. (2015) “The Influence of Humic Acids on Research in Molecular Ecology”, in “Fulvic and Humic Acids: Chemical Composition, Soil Applications and Ecological Effects”, edited by K. D. Barrett, Nova Science Publishers, New York.

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